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Annexure for Decontamination Autoclave

The autoclave is most commonly used for the decontamination of the laboratory. It works on killing the micro-organism by applying high-temperature steam per predetermine criteria. The attached annexure for the different processes is given below:

Equipment:    Decontamination Autoclave                                  Process:   Vacuum leak test
Annexure-1

Set parameters

1Pre Vacuum-0.700
2Delay before hold3 Min
3 Vacuum hold time10 min
4Acceptable leakage0.013 bar
5Process end pressure-0.060 Bar
Equipment:    Decontamination Autoclave                                  Process:   Bowie Dick Test
Annexure-1
Set Parameter
1Pre-vacuum-0.600 Bar
2Pre pressure0.600 Bar.
3Number of pre pulses3 Nos
4Heat up-1110.0 ˚C
5Heat up-2115.0˚C
6Heat up-3119.0˚C
7Heat up hold-15 Min
8Heat up hold-24 Min
9Heat up hold-33 Min
10Heat up band0.5˚C
11Small valve SP120.0˚C
12Ster. Hold temperature121.4˚C
13Ster hold time600 sec
14Temperature control band0.2˚C
15Overshoot temperature124.0˚C
16Ster. Stop temperature120.0˚C
17Ster. Reset temperature119.0˚C
18 Exhaust “ON” time7 Sec
19Exhaust “OFF” time55 Sec
20Process end pressure0.015 Bar
21Process end temp.100.0˚C
22Chamber pressure high1.500 Bar
Equipment:    Decontamination Autoclave                                      Process:  HPHV Test
Set parameters
1Pre-vacuum-0.500 Bar
2Pre pressure0.500 Bar.
3Number of pre pulses3 Nos
4Heat up-1100.0 ˚C
5Heat up-2110.0˚C
6Heat up-3119.0˚C
7Heat up hold-15 Min
8Heat up hold-24 Min
9 Heat up hold-33 Min
10Heat up band0.5 ˚C
11Small valve SP120.0˚C
12Ster. Hold temperature121.4˚C
13Ster hold time30 Min
14Temperature control band0.2˚C
15Overshoot temperature124.0˚C
16Ster. Stop temperature120.0˚C
17Ster. Reset temperature119.5˚C
18Post Vacuum start pressure0.500 Bar
19Post Vacuum-0.500 Bar
20Post vacuum hold time20 Min
21Post pressure-0.100 Bar
22Number of post pulses1 Nos
23Exhaust “ON” time7 Sec
24Exhaust “OFF” time55 Sec
25Process end pressure-0.068 Bar
26Process end temp.100˚C 
27Chamber pressure high1.500 Bar
Equipment:    Decontamination Autoclave                                     Process: Standard Process
annexure 1
Set Parameter
1Pre-vacuum0.000Bar
2Pre pressure0.000 Bar.
3Number of pre pulses0 Nos
4Heat up-1110.0 ˚C
5Heat up-2115.0˚C
6Heat up-3119.0˚C
7Heat up hold-17 Min
8Heat up hold-212 Min
9Heat up hold-315 Min
10Small valve SP120.0˚C
11Heat up band0.5˚C
12Ster. Hold temperature121.4˚C
13Ster hold time30 Min
14Temperature control band0.2˚C
15Overshoot temperature124.0˚C
16Ster. Stop temperature120.0˚C
17Ster. Reset temperature119.0˚C
18Exhaust “ON” time5 Sec
19Exhaust “OFF” time55 Sec
20Process end pressure0.050 Bar
21Process end temp.105.0˚C
22Chamber pressure high1.500 Bar
Equipment:    Decontamination Autoclave                                     Process: Standard Process
Annexure-1
Set Parameter
1Pre-vacuum0.000 Bar
2Pre pressure0.000 Bar.
3Number of pre pulses0 Nos
4Heat up-1110.0 ˚C
5Heat up-2115.0˚C
6Heat up-3119.0˚C
7Heat up hold-17 Min
8Heat up hold-25 Min
9Heat up hold-33 Min
10Small valve SP120.0˚C
11Heat up band0.5˚C
12Ster. Hold temperature121.4˚C
13Ster hold time30 Min
14Temperature control band0.2˚C
15Overshoot temperature124.0˚C
16Ster. Stop temperature120.0˚C
17Ster. Reset temperature119.0˚C
18Exhaust “ON” time5 Sec
19Exhaust “OFF” time55 Sec
20Process end pressure0.050 Bar
21Process end temp.105.0˚C
22Chamber pressure high1.500 Bar

LOG BOOK FOR DECONTAMINATION AUTOCLAVE

log book for decontamination autoclave
    by
  • Naresh Bhakar
    (Author)

    Naresh Bhakar is the Founder and Author at Pharmaguddu.com, bringing his extensive expertise in the field of pharmaceuticals to readers worldwide. He has exprience in Pharma manufactring and worked with top Pharmaceuticals. He has rich knowledge and provides valuable insights and data through his articles and content on Pharmaguddu.com. For further inquiries or collaborations, please don't hesitate to reach out via email at [email protected].

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