The autoclave is most commonly used for the decontamination of the laboratory. It works on killing the micro-organism by applying high-temperature steam per predetermine criteria. The attached annexure for the different processes is given below:
Equipment: Decontamination Autoclave Process: Vacuum leak test
Annexure-1
Set parameters
1
Pre Vacuum
-0.700
2
Delay before hold
3 Min
3
Vacuum hold time
10 min
4
Acceptable leakage
0.013 bar
5
Process end pressure
-0.060 Bar
Equipment: Decontamination Autoclave Process: Bowie Dick Test
Annexure-1
Set Parameter
1
Pre-vacuum
-0.600 Bar
2
Pre pressure
0.600 Bar.
3
Number of pre pulses
3 Nos
4
Heat up-1
110.0 ˚C
5
Heat up-2
115.0˚C
6
Heat up-3
119.0˚C
7
Heat up hold-1
5 Min
8
Heat up hold-2
4 Min
9
Heat up hold-3
3 Min
10
Heat up band
0.5˚C
11
Small valve SP
120.0˚C
12
Ster. Hold temperature
121.4˚C
13
Ster hold time
600 sec
14
Temperature control band
0.2˚C
15
Overshoot temperature
124.0˚C
16
Ster. Stop temperature
120.0˚C
17
Ster. Reset temperature
119.0˚C
18
Exhaust “ON” time
7 Sec
19
Exhaust “OFF” time
55 Sec
20
Process end pressure
0.015 Bar
21
Process end temp.
100.0˚C
22
Chamber pressure high
1.500 Bar
Equipment: Decontamination Autoclave Process: HPHV Test
Set parameters
1
Pre-vacuum
-0.500 Bar
2
Pre pressure
0.500 Bar.
3
Number of pre pulses
3 Nos
4
Heat up-1
100.0 ˚C
5
Heat up-2
110.0˚C
6
Heat up-3
119.0˚C
7
Heat up hold-1
5 Min
8
Heat up hold-2
4 Min
9
Heat up hold-3
3 Min
10
Heat up band
0.5 ˚C
11
Small valve SP
120.0˚C
12
Ster. Hold temperature
121.4˚C
13
Ster hold time
30 Min
14
Temperature control band
0.2˚C
15
Overshoot temperature
124.0˚C
16
Ster. Stop temperature
120.0˚C
17
Ster. Reset temperature
119.5˚C
18
Post Vacuum start pressure
0.500 Bar
19
Post Vacuum
-0.500 Bar
20
Post vacuum hold time
20 Min
21
Post pressure
-0.100 Bar
22
Number of post pulses
1 Nos
23
Exhaust “ON” time
7 Sec
24
Exhaust “OFF” time
55 Sec
25
Process end pressure
-0.068 Bar
26
Process end temp.
100˚C
27
Chamber pressure high
1.500 Bar
Equipment: Decontamination Autoclave Process: Standard Process
annexure 1
Set Parameter
1
Pre-vacuum
0.000Bar
2
Pre pressure
0.000 Bar.
3
Number of pre pulses
0 Nos
4
Heat up-1
110.0 ˚C
5
Heat up-2
115.0˚C
6
Heat up-3
119.0˚C
7
Heat up hold-1
7 Min
8
Heat up hold-2
12 Min
9
Heat up hold-3
15 Min
10
Small valve SP
120.0˚C
11
Heat up band
0.5˚C
12
Ster. Hold temperature
121.4˚C
13
Ster hold time
30 Min
14
Temperature control band
0.2˚C
15
Overshoot temperature
124.0˚C
16
Ster. Stop temperature
120.0˚C
17
Ster. Reset temperature
119.0˚C
18
Exhaust “ON” time
5 Sec
19
Exhaust “OFF” time
55 Sec
20
Process end pressure
0.050 Bar
21
Process end temp.
105.0˚C
22
Chamber pressure high
1.500 Bar
Equipment: Decontamination Autoclave Process: Standard Process