Annexure for Decontamination Autoclave

The autoclave is most commonly used for the decontamination of the laboratory. It works on killing the micro-organism by applying high-temperature steam per predetermine criteria. The attached annexure for the different processes is given below:

Equipment:    Decontamination Autoclave                                  Process:   Vacuum leak test
Annexure-1

Set parameters

1Pre Vacuum-0.700
2Delay before hold3 Min
3 Vacuum hold time10 min
4Acceptable leakage0.013 bar
5Process end pressure-0.060 Bar
Equipment:    Decontamination Autoclave                                  Process:   Bowie Dick Test
Annexure-1
Set Parameter
1Pre-vacuum-0.600 Bar
2Pre pressure0.600 Bar.
3Number of pre pulses3 Nos
4Heat up-1110.0 ˚C
5Heat up-2115.0˚C
6Heat up-3119.0˚C
7Heat up hold-15 Min
8Heat up hold-24 Min
9Heat up hold-33 Min
10Heat up band0.5˚C
11Small valve SP120.0˚C
12Ster. Hold temperature121.4˚C
13Ster hold time600 sec
14Temperature control band0.2˚C
15Overshoot temperature124.0˚C
16Ster. Stop temperature120.0˚C
17Ster. Reset temperature119.0˚C
18 Exhaust “ON” time7 Sec
19Exhaust “OFF” time55 Sec
20Process end pressure0.015 Bar
21Process end temp.100.0˚C
22Chamber pressure high1.500 Bar
Equipment:    Decontamination Autoclave                                      Process:  HPHV Test
Set parameters
1Pre-vacuum-0.500 Bar
2Pre pressure0.500 Bar.
3Number of pre pulses3 Nos
4Heat up-1100.0 ˚C
5Heat up-2110.0˚C
6Heat up-3119.0˚C
7Heat up hold-15 Min
8Heat up hold-24 Min
9 Heat up hold-33 Min
10Heat up band0.5 ˚C
11Small valve SP120.0˚C
12Ster. Hold temperature121.4˚C
13Ster hold time30 Min
14Temperature control band0.2˚C
15Overshoot temperature124.0˚C
16Ster. Stop temperature120.0˚C
17Ster. Reset temperature119.5˚C
18Post Vacuum start pressure0.500 Bar
19Post Vacuum-0.500 Bar
20Post vacuum hold time20 Min
21Post pressure-0.100 Bar
22Number of post pulses1 Nos
23Exhaust “ON” time7 Sec
24Exhaust “OFF” time55 Sec
25Process end pressure-0.068 Bar
26Process end temp.100˚C 
27Chamber pressure high1.500 Bar
Equipment:    Decontamination Autoclave                                     Process: Standard Process
annexure 1
Set Parameter
1Pre-vacuum0.000Bar
2Pre pressure0.000 Bar.
3Number of pre pulses0 Nos
4Heat up-1110.0 ˚C
5Heat up-2115.0˚C
6Heat up-3119.0˚C
7Heat up hold-17 Min
8Heat up hold-212 Min
9Heat up hold-315 Min
10Small valve SP120.0˚C
11Heat up band0.5˚C
12Ster. Hold temperature121.4˚C
13Ster hold time30 Min
14Temperature control band0.2˚C
15Overshoot temperature124.0˚C
16Ster. Stop temperature120.0˚C
17Ster. Reset temperature119.0˚C
18Exhaust “ON” time5 Sec
19Exhaust “OFF” time55 Sec
20Process end pressure0.050 Bar
21Process end temp.105.0˚C
22Chamber pressure high1.500 Bar
Equipment:    Decontamination Autoclave                                     Process: Standard Process
Annexure-1
Set Parameter
1Pre-vacuum0.000 Bar
2Pre pressure0.000 Bar.
3Number of pre pulses0 Nos
4Heat up-1110.0 ˚C
5Heat up-2115.0˚C
6Heat up-3119.0˚C
7Heat up hold-17 Min
8Heat up hold-25 Min
9Heat up hold-33 Min
10Small valve SP120.0˚C
11Heat up band0.5˚C
12Ster. Hold temperature121.4˚C
13Ster hold time30 Min
14Temperature control band0.2˚C
15Overshoot temperature124.0˚C
16Ster. Stop temperature120.0˚C
17Ster. Reset temperature119.0˚C
18Exhaust “ON” time5 Sec
19Exhaust “OFF” time55 Sec
20Process end pressure0.050 Bar
21Process end temp.105.0˚C
22Chamber pressure high1.500 Bar

LOG BOOK FOR DECONTAMINATION AUTOCLAVE

log book for decontamination autoclave

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