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Procedure for Microbial Incubator operation & Calibration

Learn About Standard Operating Procedure for Microbial Incubator operation and their Calibration along with Objective, Scope, Responsibility, Procedure, Frequency, and Tolerance Limits

1.0 Objective: To get reliable and consistent results, we must ensure the procedure and maintenance of the Microbial Incubator, especially for temperature maintenance for Microbial growth.

2.0 Scope: This procedure is applicable to the Microbiology Department.

File with title on Procedure for Microbial Incubator operation & Calibration

3.0 Responsibility:
The responsibility is with the Microbiologist.
The Q.A. Manager carried out the operation.

4.0 Procedure:
4.1 Ensure that the equipment is free from dust.
4.2 Switch on the main and equipment.
4.3 Set the incubator temperature by pressing the red color button manually on display for about 35°C using Coarse and Fine knobs.
4.4 Place a thermometer in the hole provided on the top of the equipment.
4.5 Open the door and acrylic sheet of the Incubator.
4.6 Place the plates on the incubator shelf in the inverted position.
4.7 Close the acrylic sheet and the door of the Incubator.
4.8 Maintain the same temperature throughout the five days of the incubation period.
4.9 At the end of the day, count the colonies, and the record should be maintained properly.

Related SOP: Procedure for Hot Air Oven

5.0 Calibration Procedure of Microbial Incubator:
5.1 Place a calibrated thermometer on the Top, Middle, and Bottom of the equipment and close the door.
5.2 Manually set the temperature on display once at 35°C. After 30 minutes, record the thermometer reading.
5.3 Continue the above second procedure for 40°C and 50°C. After 30 minutes, record the thermometer readings.

6.0 Troubleshooting problems and remedial action:
6.1 Suppose The temperature display is not working or glowing for this first check the electric connection and power supply.
6.2 Temperature is not even in the Incubator. The air-circulating fan may not be functioning. Check for it.
6.3 Report any discrepancies observed during operation or temperature monitoring to the Q.C. Executive and notify the defect to Maintenance Department.

Frequency of Calibration: Once a Year.
In-house calibration as per frequency or on a monthly basis

Tolerance limit: ±2°C 

Related SOP: Procedure for BOD incubator

SOP- Standard operating procedure
Q.C. – Quality Control

  • Naresh Bhakar

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