Home » Pharma SOPs » QC SOPs » SOP for estimation of Nickel (IS 12122 -1987)

SOP for estimation of Nickel (IS 12122 -1987)

Standard working procedure for estimation of Nickel / Standard Working procedure for Nikel estimation by using AAS Methods.

Principle:

The Nickel content in the sample is determined by aspirating the sample into the flame of an Atomic Absorption Spectrophotometer after pre-concentration. Measured the Absorbance at 232 nm using a Nickel Hollow Cathode Lamp.

Limit of Detection: The limit of detection is 0.01 mg/L.

Reagents used for estimation of Nickel:

  • Concentrated HCL
  • Concentrated Nitric Acid
  • Dilute Nitric Acid (1:499)
  • Nickel Stock Solution: Merck 1000 mg/L NIST (National Institute of Standards and Technology) Certified Reference Material or 1.0 gm of pure metal dissolved in concentrated HNO3, heated for complete dissolution. After cooling, transfer quantitatively to a 1000 ml volumetric flask, dilute to the mark with water, and mix. (1 ml of standard solution contains 1 mg of Ni)

Procedure for estimation of Nickel:

Preparation of Standard Solution:

Prepare a reagent blank and a series of 100 ml standards containing 0.1, 0.2, 0.3, 0.4, and 0.5 mg/L of Nickel by diluting a suitable volume of the standard solution with dilute nitric acid.

Sample Preparation:

  • Take a 1000 ml well-mixed sample, transfer it to the evaporation dish, and add 3 ml nitric acid.
  • Heat and evaporate to 20 ml (avoid boiling).
  • Cool, add 3 ml nitric acid, and heat until digestion is complete.
  • Add 2 ml HCl (1:1 v/v) and heat gently to dissolve the residue.
  • Wash the evaporation dish 3 to 4 times with water, filter, and discard the filter.
  • Dilute the filtrate to make up a 100 ml volume with water. (Sample is concentrated 10 times)

Analysis:

  • Operate the instrument by following the Standard procedure.
  • Aspirate dilute nitric acid (1:499) as a blank.
  • Aspirate a series of standards and then the sample solution.
  • Read the concentration of Nickel directly in mg/L.
  • Apply the dilution factor of 0.1 due to the sample being concentrated 10 times.

Reference: IS 12122 (1987)Latest

Sharing Is Caring:

Leave a Comment