SOP for estimation of Nickel (IS 12122 -1987)

Standard working procedure for estimation of Nickel / Standard Working procedure for Nikel estimation by using AAS Methods.


The Nickel content in the sample is determined by aspirating the sample into the flame of an Atomic Absorption Spectrophotometer after pre-concentration. Measured the Absorbance at 232 nm using a Nickel Hollow Cathode Lamp.

Limit of Detection: The limit of detection is 0.01 mg/L.

Reagents used for estimation of Nickel:

  • Concentrated HCL
  • Concentrated Nitric Acid
  • Dilute Nitric Acid (1:499)
  • Nickel Stock Solution: Merck 1000 mg/L NIST (National Institute of Standards and Technology) Certified Reference Material or 1.0 gm of pure metal dissolved in concentrated HNO3, heated for complete dissolution. After cooling, transfer quantitatively to a 1000 ml volumetric flask, dilute to the mark with water, and mix. (1 ml of standard solution contains 1 mg of Ni)

Procedure for estimation of Nickel:

Preparation of Standard Solution:

Prepare a reagent blank and a series of 100 ml standards containing 0.1, 0.2, 0.3, 0.4, and 0.5 mg/L of Nickel by diluting a suitable volume of the standard solution with dilute nitric acid.

Sample Preparation:

  • Take a 1000 ml well-mixed sample, transfer it to the evaporation dish, and add 3 ml nitric acid.
  • Heat and evaporate to 20 ml (avoid boiling).
  • Cool, add 3 ml nitric acid, and heat until digestion is complete.
  • Add 2 ml HCl (1:1 v/v) and heat gently to dissolve the residue.
  • Wash the evaporation dish 3 to 4 times with water, filter, and discard the filter.
  • Dilute the filtrate to make up a 100 ml volume with water. (Sample is concentrated 10 times)


  • Operate the instrument by following the Standard procedure.
  • Aspirate dilute nitric acid (1:499) as a blank.
  • Aspirate a series of standards and then the sample solution.
  • Read the concentration of Nickel directly in mg/L.
  • Apply the dilution factor of 0.1 due to the sample being concentrated 10 times.

Reference: IS 12122 (1987)Latest

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