Dilution Calculator for Pharmaceutical

The Dilution Calculator allows pharmaceutical professionals, microbiologists, laboratory analysts, students, and quality control personnel to easily calculate the volume of stock solution needed to make a solution of a desired concentration.

Dilution Calculator
Per WHO GMP / ICH Q7 Guidelines
✓ GMP Verified Free Tool
ℹ
Original concentration of the stock solution.
mg/mL
ℹ
Target concentration after dilution.
mg/mL
ℹ
Required total volume of diluted solution.
mL
Result
mL
Formula used:
For calculation assistance only. Verify against your SOP.

Dilution Formula used

The calculator uses the standard dilution equation:

C₁V₁ = C₂V₂

C₁ Initial (stock) concentration
V₁ Volume of stock solution to be taken (mL)
C₂ Desired concentration
V₂ The final volume needed

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Frequently Asked Questions

Q. What is the dilution formula?

Ans: The general formula for the dilutions is: C₁V₁ = C₂V₂.

Q. Can this calculator be used for pharmaceutical applications?

Ans: Yes. The calculator can be used for pharmaceutical, microbiological, analytical, educational and research use when dilutions are required.

Q. What happens if the desired concentration is greater than the stock concentration?

Ans: If the stock concentration is less than the target concentration, a dilution cannot be done. In such cases, a more concentrated stock solution is required.

Q. How to determine the volume of diluent?

Ans: Subtract the volume of calculated stock solution from the final volume:
Diluent Volume = Final Volume – Stock Solution Volume

Q. Can this calculator be used for serial dilutions?

Ans: This calculator can be used to do one dilution calculation. The calculation needs to be repeated for each dilution step for serial dilution studies.