The major difference between HPLC and LCMS is that HPLC uses Non-volatile and Volatile buffers whereas LCMS uses Only volatile buffers. Common Difference between HPLC and LCMS/HPLC-MS HPLC full form is High-Performance Liquid Chromatography. It is a technique that is used to separate and analyze compounds in a mixture using liquid chromatography. It is a … Read more
Lead is a most undesirable impurity in pharma compounds and comes through the use of sulphuric acid, lead-lined apparatus, and glass bottles used for the storage of chemical materials so, we do a Limit Test for Lead to identify the acceptance level of lead in a sample. Limit test for lead Principle The limit test … Read more
The limit test for arsenic is based on the reduction of the arsenic in the arsenic state to the arsenic gas with zinc and hydrochloric acid. The arsenic gas stains the mercuric chloride paper yellow. The paper is dissolved in acid where the arsenic present as an impurity in the sample gets converted into arsenic … Read more
The Limit Test for Sulphate is designed to determine the allowable limit of Sulphate contained in a sample. Principle: The principle for the limit test of Sulphate is based on the reaction of soluble Sulphate with barium chloride in the presence of dilute hydrochloric acid to form barium Sulphate which appears as turbidity with a … Read more
A limit test for chloride is done to identify the impurity in the compounds by comparing the test solution against the standard solution. The chloride limits test is only designed to identify and control the impurity present in the compounds. Limit Test for Chloride Principle The limit test for chloride is based on the reaction … Read more
The limit test for iron refers to a type of analytical chemistry test used to determine the concentration of iron in a sample. This test is often performed in the laboratory as part of a larger suite of tests to determine the chemical composition of a substance.In a limit test for iron, a known volume … Read more
In pharmaceutical analysis, a limit test is a type of analytical test used to determine impurity in a substance whether a substance is present in a sample at a concentration above or below a specified limit. Limit tests are often used to ensure that a substance meets certain quality standards or regulatory requirements. Definition The … Read more
Quality control equipment is widely used in pharmaceuticals for the testing of drugs as per given standards. The subject of analytical instruments has grown more complex recently. In order to get more precise results, personal computers and microcontrollers have been added to analytical equipment in modern times. A chosen few of these instruments are also … Read more
Various types of HPLC columns from the analytical point of view. know about what is an HPLC column? The column is the heart of the HPLC. The column stationary phase separates the sample components of interest using various physical and chemical parameters. It is usually made of stainless steel to withstand high pressure caused by … Read more
Aim: Methyl Orange Indicator Preparation | How to make w/v methyl orange Requirements: Glasswares: Burette, burette stand, conical flask, volumetric pipette, beaker, measuring flask, funnel, glass rod, wash bottle, storage container, etc. Chemicals: Methyl orange, Ethanol 20 ml, and Distilled water. Procedure for Methyl Orange Indicator preparation Related Post: Preparation and Standardization of 0.1 N HCl
To Preparation and Standardization of 0.1 N HCl utilizing sodium carbonate (also comprehended as washing soda, soda ash, and soda crystals) as the primary standard (Acid-base titration). Prerequisites for preparation and standardization of 0.1 N HCL: Glasswares: Burette, stand for burette, a conical flask, volumetric pipette to makeup volume, beaker, volumetric flask, funnel, glass rod, … Read more
Both humidity and relative humidity refer to the amount of water vapour in the air, however, they are not the same. Humidity is the absolute amount of water vapour in the air, whereas relative humidity is the amount of water vapour in the air in comparison to the maximum amount of water vapour the air might … Read more
Sometimes we may confused when it comes to understanding the difference between moisture content and Loss on Drying (LOD). To clear your doubts, please read the entire section of this post. Firstly, let’s discuss about moisture analyzers. A moisture analyzer is used to determine the water content in a sample. Then a few questions arise … Read more
pH meter is used to measure the acidity and alkalinity of a solution by measuring Hydrogen ion concentration in a solution. pH Meter Calibration is required at regular intervals or as per the defined frequency to maintain its accuracy. Here, we will discuss the pH meter principle, ph meter electrode, Range of pH, Calibration, Key … Read more
Good Laboratory Practice (GLP) is a set of guidelines that helps ensure the quality and reliability of pharmaceutical laboratory data. These GLP guidelines are important because they help to prevent errors and they also ensure that data collected during laboratory testing is accurate and consistent. Understanding GLP guidelines in Pharmaceuticals: Good Laboratory Practice (GLP) guidelines … Read more
High-Performance Liquid Chromatography (HPLC) is used in pharmaceuticals for the analysis of drug mixture and their components. HPLC is reverse-phase Liquid Chromatography. This technique is used for thermal labile, high molecular weight peptides, and non-volatile compounds. The main purpose of HPLC is used to examine the mixture for its identification, impurities, and purification. The mixture … Read more