Degassing of HPLC mobile phase removes dissolved air so bubbles do not form when solvents mix. Bubbles cause false peaks, noisy baselines, lower pump flow rate precision and possible pump failure. Helium purging, vacuum degassing and sonication are the common methods, and combining them works better than using one alone. Why is degassing of HPLC … Read more
Preparation and Standardization of 0.1 N Perchloric acid (Non-Aquos by using glacial acetic acid and crystal violet solution as indicators in Pharmaceuticals. Apparatus and Reagents Requirements: Apparatus: Conical Flask, Volumetric Flask, Reflux condenser, Measuring cylinder, Burette, Pipette and Burette Stand. Reagents: Preparation of 0.1 N Perchloric acid Standardization of 0.1 N Perchloric acid Calculation: N1V1=N2V2 … Read more
Here is a detailed discussion on How to Prepare and Standardization of Potassium Permanganate (1M, 0.1M, 1N, 0.1N) solution by using different reagents in Labs. Preparation and Standardization of 1M Potassium Permanganate Potassium permanganate – M.Wt – 158 Preparation of 1M Solution of Potassium Permanganate: Principle: In volumetric analysis, many reactions involve oxidation and reduction. … Read more
Preparation and Standardization of 0.1 M Sodium Thiosulfate (Na2S2O3) solution in pharmaceutical labs. Aim: 0.1 Molar Thiosulfate (Na2S2O3) solution Preparation and Standardization Materials/Reagents: 1.0 – Sodium Thiosulfate2.0 – Sodium Carbonate3.0 – Potassium Bromate4.0 – Potassium Iodide5.0 – Hydrochloric Acid6.0 – Starch Solution Principle: Principle: This method uses a standard process called redox titration with iodometric … Read more
Preparation and Standardization of 0.1 M KOH Solution by using HCL and phenolphthalein as an indicator in Pharmaceutical Labs. Aim: To Prepare and measure 0.1 M KOH solution with HCl. Materials and Reagents Requirements: Glass: A burette, burette stand, conical flask, volumetric pipette, beaker, volumetric flask, funnel, glass rod, and a wash bottle.Chemicals: Potassium hydroxide … Read more
Preparation and Standardization of 0.1N Silver Nitrate (AgNO3) Solution in pharmaceutical labs by using below steps: Steps involved in the Preparation and Standardization of 0.1N Silver Nitrate Step-1: Reagent preparationStep-2: AgNO3 solution preparationStep-3: AgNO3 solution StandardizationStep-4: Calculation Preparation of 0.1 N AgNO3 solution: Step-1: Reagent Preparation: (Pottasium Chromate solution) Step-2: AgNO3 solution preparation: Step-3: AgNO3 … Read more
Aim: Preparation and Standardization of 0.1 M Silver Nitrate (AgNO3) solution by using sodium chloride in Pharmaceutical Labs. Name: 0.1 M Silver Nitrate (AgNO3) Reagents used to Prepare and Standardization of 0.1 M AgNO3 : Glassware: Volumetric Flask, measuring cylinder, Burette, Conical Flask, beaker, and Pipettes. Principle: In the lab, we used a process where … Read more
Preparation and Standardization of 0.1 M Sodium Nitrite (NaNO2) by using Sulphanilic acid in Pharmaceutical Analysis. Chemical Name: 0.1 M Sodium Nitrite (NaNO2) Reagents: 1.0 Sodium nitrite2.0 Sulphanilic acid3.0 Potassium bromide4.0 2M Hydrochloric Acid5.0 Starch iodide paper Preparation of 0.1 M Sodium Nitrite To make this solution, take 7.5 grams of sodium nitrite and dissolve … Read more
Preparation and Standardization of 0.05 M EDTA Solution by using disodium edetate and Eri Chrom Black T as an indicator in Pharmaceutical Labs. EDTA, which stands for Edathamil, or Ethylene diamine tetra acetic Acid, is a well-known substance used in managing and treating heavy metal toxicity. Preparation of 0.05 M EDTA Solution: To prepare a … Read more
Aim: To Prepare and standardize 0.05 M Iodine Solution by using As2O3 Name: 0.05 M Iodine solution Ingredients Required for Preparation and Standardization of 0.05 M Iodine Solution: How to Preparation and Standardization of 0.05 M Iodine Solution To Prepare this solution, you’ll need to follow these steps: How to Standardization of 0.05 M Iodine … Read more
Aim: The aim is to find out the normality of the given 0.1 M Ceric ammonium Sulphate. Requirements: Equipment: A special tube with a tap, a cone-shaped flask, and a small tube.Reagents: Ceric ammonium sulphate, Sulphuric acid, Arsenic trioxide, Sodium hydroxide, and some more sulphuric acid. Principle: We test Ceric ammonium sulphate by mixing it … Read more
Name: How to Preparare and Standardization of 0.1 M Disodium Edetate (EDTA Ingredients : 1. Disodium Edetate. 2. Calcium Carbonate 3. Eriochrome black – T 4. Ammonia – Ammonium Chloride Buffer pH10 (Mix 5.4 grams of NH4Cl + 35 milliliters of 10M NH3 solution & dilute to 100 milliliters with water.) 5 Hydrochloric Acid. How … Read more
Aim: To prepare and standardize 0.5 M sulfuric acid (0.5 M H2SO4). Principle: 1. This is carried out by aqueous acid-base titration based on a neutralization reaction. 2. This titration takes place between sulphuric acid (H2SO4) and sodium carbonate (Na2CO3). 3. Sodium carbonate, anhydrous is a primary standard substance that reacts with a secondary standard … Read more
Objective: The purpose of this experiment is to standardize 1.0 M Sulfuric Acid. Materials used for 1.0 M Sulfuric Acid: Equipment: Beaker, Funnel, Pipette, BuretteChemicals: H2SO4, Phenolphthalein solution, and Anhydrous sodium carbonate. Theory: Methyl orange is commonly used as an indicator in titrations. In an alkaline solution, methyl orange appears yellow and has the following … Read more
Preparation and Standardization of 0.1 M Sodium Hydroxide (NaOH) in the Laboratory. Reagents: 1 Sodium hydroxide 2 Potassium hydrogen phthalate 3 Phenolphthalein solution. Chemical Reaction Principle: In this process, we will directly measure the strength of potassium hydrogen phthalate by using sodium hydroxide. To find the endpoint, we’ll use phenolphthalein as an indicator. Let’s understand … Read more
In this experiment, we will focus on the preparation and standardization of a 1 M Sodium Hydroxide solution. Molarity and How to Prepare 1 M NaOH with Example: Example for factor calculation for standardization of 1 m NaOH:Na=23O=16H=1Total= 40 gm. Reagents Required for Preparation and Standardization of 1 M NaOH: 1 Sodium Hydroxide 2 Potassium … Read more